%0 Journal Article
%A CAO Lu-Yao
%A JIN Zhi-Nong
%A LI Feng
%A LIAO Wen-Xuan
%A Lü Xiong-Wei
%A WANG Ling-Ling
%A XIAO Zu-Fei
%A ZHANG Bei-Hong
%A ZHAO Jiao
%T Tissue Culture Technology of Stem Segment of <i>Cinnamomum bodinieri</i> var. <i>citralifera</i>
%D 2020
%R 10.7525/j.issn.1673-5102.2020.02.005
%J Bulletin of Botanical Research
%P 196-201
%V 40
%N 2
%X <i>Cinnamomum bodinieri</i> var. <i>citralifera</i> is an important tree species for landscaping, timber and oil. We studied the effects of collection season, disinfection time and hormones on tissue culture of annual branches of <i>C.bodinieri</i> var. <i>citralifera</i>. The axillary bud germination rate of stem segments from semi-lignified stem segments of <i>C.bodinieri</i> var. <i>citralifera</i> was highest with 0.1% HgCl<sub>2</sub> disinfected for 5 min. in the Mid-May, and the browning rate and pollution rate were low. The suitable medium for stem segment germination was MS+1.0 mg&#183;L<sup>-1</sup> 6-BA+0.05 mg&#183;L<sup>-1</sup> IBA, with germination ratio 60%. The suitable medium for multiplication was MS+1.0 mg&#183;L<sup>-1</sup> 6-BA+0.2 mg&#183;L<sup>-1</sup> IBA, with multiplication coefficient of 4.33, plant height of 3.67 cm and ground diameter of 1.04 mm. Adventitious roots appeared at 11 d after culture of the base of tissue culture seedlings. The suitable medium for rooting was MS+1.5 mg&#183;L<sup>-1</sup> IBA. The rooting rate was 75.00%, the length of roots was 5.06 cm, the number of roots was 3.50, and the diameter of roots was 0.82 mm. Tissue culture seedlings with roots more than 3 had a survival rate of more than 80%.
%U https://bbr.nefu.edu.cn/EN/10.7525/j.issn.1673-5102.2020.02.005